DBCO-PEG10000-Silane 二苯并环辛炔-聚乙二醇-硅烷含有硅烷和二苯并环辛炔的PEG-UDP糖丨MOF丨金属有机框架丨聚集诱导发光丨推荐西安齐岳生物 -

银催化条件下，DBCO可以快速的酰化氨基官能团；对三种包含20种天然氨基酸的多肽底物研究发现，DBCO酰化反应高选择性地发生在Lys的氨基上，而不影响其他氨基酸。同时，银催化DBCO酰化反应可以**地修饰糖肽、BSA蛋白、糖蛋白RNase B 和IgG抗体等，表现出较好的底物适用性。此外，银催化酰化反应可以实现Ph.D.12商业噬菌体库的修饰且不影响噬菌体侵染活力，说明该方法具有应用于活生物体系中的潜力。

在反应缓冲液中制备含叠氮化物的样品。将DBCO共轭物添加到含叠氮化物的样品中。建议：在丰度试剂的1.5-3.0摩尔当量中添加1摩尔当量的限制试剂。将反应在室温下于4摄氏度温育2-4小时或2-12小时。现在可以将反应纯化。

DBCO is used for copper free click chemistry. Without copper catalyst, the reaction with azide compound is very rapid. This reaction is very rapid, mild, and biocompatible.

Under silver catalysis, DBCO can quickly acylate amino groups; The study of three peptide substrates containing 20 natural amino acids found that the DBCO acylation reaction occurred on the amino acid of Lys with high selectivity, without affecting other amino acids. At the same time, silver catalyzed DBCO acylation reaction can efficiently modify glycopeptides, BSA proteins, glycoprotein RNase B and IgG antibodies, showing good substrate applicability. In addition, silver catalyzed acylation can achieve the modification of commercial phage library of Ph.D.12 without affecting the infection activity of phages, indicating that this method has potential application in living biological systems.

The sample containing azide was prepared in the reaction buffer. DBCO conjugate was added to the sample containing azide. It is recommended to add a limiting reagent of 1 mole equivalent to the 1.5-3.0 mole equivalent of the abundance reagent. The reaction was incubated at 4 ℃ at room temperature for 2-4 hours or 2-12 hours. The reaction can now be purified.

DBCO-GPC二苯基环辛炔-甘磷酸胆碱

DBCO-PFP二苯基环辛炔-五氟苯酚

DBCO-Benzaldehyde二苯基环辛炔-苯甲醛

DBCO-Mesylate 二苯基环辛炔-甲磺酸酯

DBCO-TPP二苯基环辛炔-磷酸三苯酯

DBCO-Phenyl二苯基环辛炔-苯基

DBCO-acid二苯基环辛炔-磷酸

DBCO-GA二苯基环辛炔修饰甘草次酸

DBCO-Cyclodextrin二苯基环辛炔偶联环糊精

DBCO-Dopamine 二苯基环辛炔修饰多巴胺

DBCO-Ferrocene二苯基环辛炔修饰二茂铁

用途:科研